EVALUATION OF THE EFFECT OF ILEX PARAGUARIENSIS EXTRACT ON CELL PROLIFERATION.

MARIA ROSANA RAMIREZ; AGOSTINA GUZZONATTO; IGNACIO RINTOUL

Mar del Plata

Congreso; REUNIÓN CONJUNTA SAIC. SAI. SAFIS | 2022; 2022

Sociedad Argentina de Investigación Clínica (SAIC), Sociedad Argentina de Inmunología (SAI) y la Sociedad Argentina de Fisiología (SAFIS).

Ilex paraguariensis (Aquifoliaceae) leaves are used for their stimulant, antioxidant, and diuretic activity, presenting as principal components polyphenolic compounds. Previous work reported that I. paraguariensis also inhibits the proliferation of oral cancer cells in vitro. Our aim was to study the effect of I. paraguariensis extract on cell proliferation in the endometrial stromal cell line (tHESC cells). For this purpose, tHESC cells were seeded in plates containing culture medium (DMEM-F12, L-glutamine, foetal bovine serum). After approximately 48 hours, the medium was removed from the plates, washed with PBS and the cells were stimulated with I. paraguariensis extract (4-400 µg/mL). The corresponding blanks (40 to 400 µg/mL) were then made. Cell proliferation was determined by a colorimetric method (450 nm and at 620 nm in a plate reader). There were 4 replicates per treatment and each experiment was carried out 5 times (n=5). In both cases an analysis of variance (ANOVA) followed by Tukey´s test was performed. Results are expressed as the mean per treatment ±SEM, p ≤ 0.05 is considered statistically significant (GraphPad Prism). The results showed that I. paraguariensis extract at doses of 100-400 µg/ml showed a statistically significant inhibition of cell proliferation compared to basal (p ≤ 0.001). It is possible to suggest that I. paraguariensis extract requires further studies to determine its efficacy on cell proliferation and safety in, in vivo studies.