ILEX PARAGUARIENSIS EXTRACT IMPROVES BIO- MECHANICAL PARAMETERS IN OLD MICE: AN IN VIVO STUDY

MARIA ROSANA RAMIREZ; VALERIA GRISELDA CEREVIN; ADRIANE JORAS MACHADO; IGNACIO RINTOUL

Mar del Plata

Congreso; ANNUAL MEETING OF BIOSCIENCE SOCIETIES 2020; 2020

Sociedad Argentina de Investi-gación Clínica, la Sociedad Argentina de Fisiología y la Sociedad Argentina de Inmunología

Tea from Ilex paraguariensis (IP) is widely available and is a good source of caffeine and other bioactive compounds. The genus Ilex, are also traditional drinks, with lesser overall usage, but have attracted much recent attention and have been subjected to further study. Because the potential of IP has been suggested in the management of obesity, the aim of the present study was to evaluate the putative effect of IP extract on weight loss, biochemical parameters in old obese mice. Thirty animals were randomly assigned to three groups according to the treatment (water or IP extract 1.0 g/kg body weight). The roasted IP extract was prepared according Pharmacopoeia, and administrated by intragastric gavage. The experiments were performed in accordance with the principles for animal experimentation. After treatment intervention (30 days), plasma concentrations of total cholesterol, high-density lipoprotein colesterol (HDL), triglyceride, and glucose were evaluated using WienerLab (Rosario, Argentina). Plasma LDL cholesterol concentration was calculated according to the formula: CHOL−(triglyceride/5 + high-density lipoprotein. Comparisons among groups of data were done using one way ANOVA followed by the Dunnett Multiple Comparisons test. An associated probability (P value) of <5% was considered significant. The results found that old mice treated with IP extract exhibited attenuation of weight gain, and restoration of the serum levels of cholesterol, triglycerides, LDL cholesterol, and glucose. In conclusion, the data show that IP extract has potent bioactivity in vivo. There are data showing that animal models are a useful tool to evaluate the efficacy of potential compounds in the prevention and treatment of obesity.