Carboxylated Latex Particles Sensitized with Antigenic Proteins of various Diseases for their Use as Immunoagglutination Reagents

GONZALEZ, V.D.G.; GARCIA, V.S.; PERETTI, L.E.; PROCHETTO, S.D.; GUGLIOTTA, L.M.

Florianópolis

Congreso; 12 Congreso Brasileiro de Polímeros; 2013

Asociación Brasileira de Polímeros

Monodisperse polymer colloids with carboxyl functionality were synthesized through a two-step emulsion polymerization process. The synthesized ?core-shell? particles were used as support for their sensitization (by covalent coupling) with antigens of Trypanosoma cruzi, Toxoplasmas gondii and Leptospira interrogans. The resulting latex-protein complexes were used in immunoagglutination assays for detecting Chagas disease, Toxoplasmosis and Leptospirosis, respectively. In all cases, high fractions of proteins chemically-bound to carboxyl groups were observed and all latex-protein complex allowed discrimination between sera from infected and non-infected patients