PERSONAL DE APOYO
ALVAREZ Guadalupe InÉs
congresos y reuniones científicas
Título:
The IL-17 rs2275913 single nucleotide polymorphism (SNP) is associated with human tuberculosis susceptibility in Argentina
Autor/es:
AGUSTÍN ROLANDELLI; RODRIGO HERNÁNDEZ DEL PINO; GUADALUPE INÉS ALVAREZ; JOAQUÍN PELLEGRINI; NICOLÁS TATEOSIAN; NANCY AMIANO ; DOMINGO PALMERO; NICOLÁS CASCO; MARISA GUTIERREZ; VIRGINIA PASQUINELLI; VERÓNICA GARCÍA
Lugar:
Buenos Aires
Reunión:
Congreso; IV Congreso de la Sociedad Latinoamericana de Inmunodeficiencias (LASID), LXIII Reunión de la Sociedad Argentina de Inmunología (SAI) y II Reunión FAIC (French-Argentinean Immunology Congress); 2015
Institución organizadora:
Sociedad Argentina de Inmunología. Sociedad Latinoamericana de Inmunodeficiencias
Resumen:
THE IL-17 rs2275913 SINGLE NUCLEOTIDEPOLYMORPHISM (SNP) IS ASSOCIATED WITH human TUBERCULOSIS SUSCEPTIBILITY inargentina.Background. Interleukin 17A (IL-17) plays animportant role in host-pathogen interaction during Mycobacterium tuberculosis (Mtb) infection. Genetic variations in IL-17 gene may affect the immune responseagainst this bacterium. The SNP rs2275913 is the most studied nucleotide substitution inIL-17A gene, but only one study reported the association ofthis SNP with tuberculosis susceptibility in a Spanish population. Here weinvestigated the relationship between rs2275913 SNP and tuberculosissusceptibility in Argentina and evaluated the functional implications of thesubstitution. Methods. 146tuberculosis patients (TB) and 164 healthy donors (HD) were genotyped byARMS-PCR. IFN-g and IL-17Alevels were analyzed by ELISA in plasma and supernatants from peripheral bloodmononuclear cells (PBMCs) stimulated with a Mtblysate. Results. Significantdifferences in the allelic and genotypic (p<0.001) distribution of the rs2275913 SNP were foundbetween TB and HD. Moreover, AA or GA individuals showed a marked lowerassociation with tuberculosis development as compared with GG subjects (OR AAvsGG=0.27, OR GAvsGG=0.48; p<0.01).Additionally, higher IL-17 levels were detected in plasma (p<0.01HD; p<0.05TB) andPBMCs supernatants (p<0.05HD; p<0.05TB)of AA individuals as compared to GG subjects. Furthermore, higher levelsof IFN-g were detected in supernatants of AA and GA HDcompared to GG donors (p<0.01). In contrast, in TB, significanthigher IFN-g levels weredetected in supernatants of GG and GA subjects (p<0.05). Thus, the IL-17 genotypes might differentially regulate the cytokine microenvironment. Conclusions. Our results suggest thatAA and GA genotypes could be important protection markers during activetuberculosis development in Argentina.