INVESTIGADORES
LAVIA graciela Ines
congresos y reuniones científicas
Título:
Prebreeding of Arachis hypogaea: development of materials from reciprocal interspecific crosses between Arachis L. species with genome B and analysis of the meiotic behavior of F1 hybrids
Autor/es:
GARCÍA, A.V.; VANDECAVEYE, M.A.; LAVIA, G.I.
Lugar:
Foz do Iguacu, PA
Reunión:
Congreso; 2018 International Congress of Genetics; 2018
Institución organizadora:
Sociedad Brasilera de Genética (SBG) y la Asociación Latinoamericana de Genética (ALAG)
Resumen:
The cultivated peanut Arachis hypogaea (AABB genome) is a crop of world economic importance. Thus, to incorporate features of interest to the crop, it is essential to develop hybrid materials from wild Arachis species. Arachis ipaënsis and A. williamsii present desirable characteristics to beincorporated into the cultigen. Although these two species are B genome, whether or not they are genomically homologous has not yet been determined by meiotic behavior. Thereby the aims of this work were to develop hybrid materials from A. ipaënsis and A. williamsii and determine the genomic homology between the parental species. The crosses were made in greenhouse by castrating female parent flowers and pollinating them with the male parent. This techniqueallowed obtaining reciprocal interspecific hybrids. SSR markers and pollen viability were employed to verify the hybrid nature of the plants. Meiotic behavior was analyzed by evaluating flower buds. Analysis of pollen mother cells at diakinesis/metaphase I revealed that 65.7% of cells had 10II. The formation of multivalents demonstrated the emergence of reciprocal translocationsbetween non-homologous chromosomes. The irregularities observed from Prophase I to Telophase II would result in the formation of aneuploid microspores. In addition, the presence of persistent anaphase bridges and tripolar spindles would be responsible for the formation of unreduced gametes. Successful hybridization allowed the development of diploid materials with high formation of bivalents and high pollen viability, which indicates that parental species have homologous genomes; consequently these are promising materials for genetic improvement programs of cultivated peanut.