PARACRINE CROSSTALK BETWEEN PERIVASCULAR ADIPOSE TISSUE AND SMOOTH MUSCLE CELLS IN FRUCTOSE FED- APOLIPOPROTEIN-DEFICIENT MICE

CEJAS JIMENA; QUESADA ISABEL; REDONDO ANALIA; SERRANO RODRIGO; CASTRO CLAUDIA

Congreso; LXI REUNIÓN ANUAL DE LA SOCIEDAD ARGENTINA DE INVESTIGACIÓN CLÍNICA (SAIC); 2016

Perivascular adipose tissue (PVAT) release growth factors and adipokines that could have direct paracrine effects on the underlying vessel. Type 2 diabetes can affect PVAT causing abnormal fat cells and infiltration of inflammatory cells, that produce an imbalance between PVAT derived factors. We set out to study the effect of PVAT secretomes obtained from C57BL/6 mice(WT), Apolipoprotein-E deficient-mice (ApoE-KO) and fructose- fed ApoE-KO mice on cultured vascular smooth muscle cells (VSMC). Secretomes were obtained from 400 mg of aortas adipose tissue in 1 ml of DMEM/F12 at 37ºC for 4 hours. Treatment with ApoEKO-PVAT secretomes showed a significant increase in VSMC proliferation compared to WT-PVAT secretomes (p