INVESTIGADORES
GOMEZ-MEJIBA sandra Esther
libros
Título:
COMPENDIUM: TECHNIQUES IN MOLECULAR GENETICS
Autor/es:
DARIO C RAMIREZ; GOMEZ MEJIBA, SANDRA;; SERGIO E. ALVAREZ; MARIA ELENA ARCE; MARIA BELEN TORRES BASSO; PILAR FERRARIS; HELGA BLANCO
Editorial:
EDITORIAL UNIVERSITARIA-UNSL
Referencias:
Lugar: SAN LUIS; Año: 2015 p. 252
ISSN:
9781111138363
Resumen:
CONTENTS1. RNA extraction using TRIZOL (Invitrogen) ..................................................................................................
..12. Determining the Yield and Quality of Purified RNA ................................................................................
..23. PCR Primer Design ....................................................................................................................................................
...104. Nondenaturing Agarose Gel Electrophoresis of RNA..............................................................................
...215. The Technique of In Situ Hybridization .........................................................................................................
...256. Array-Based Nuclear Run-On Analysis ...........................................................................................................
...497. Chromatin Immunoprecipitation Assay as a Tool for Analyzing Transcription Factor Activity...................................................................................................................................................................................................
...628. CAGE (Cap Analysis of Gene Expression): A Protocol for the Detection of Promoter andTranscriptional Networks .......................................................................................................................................... ...
829. Native Chromatin Immunoprecipitation .......................................................................................................
10210. Basic principles of real-time PCR ....................................................................................................................
12011. Optimization and Troubleshooting in PCR ................................................................................................
15612. Reverse Transcriptase Assay (RT) .................................................................................................................
16313. Detection of Small Non-coding RNAs ............................................................................................................
16414. Quantitative Estimation of Multiple miRNAs and mRNAs from a Single Cell .........................
17415. Human embryonic stem cell protocols ........................................................................................................
18616. Microarray Protocols .............................................................................................................................................
20517. Mobility Shift Assays..............................................................................................................................................
22718. Usefulness of the Luciferase Reporter System to Test the Efficacy of siRNA ..........................
23619. RNA Immunoprecipitation to Determine RNA-Protein Associations In Vivo ..........................
243

