INVESTIGADORES
GOMEZ-MEJIBA sandra Esther
libros
Título:
COMPENDIUM: TECHNIQUES IN MOLECULAR GENETICS
Autor/es:
DARIO C RAMIREZ; GOMEZ MEJIBA, SANDRA;; SERGIO E. ALVAREZ; MARIA ELENA ARCE; MARIA BELEN TORRES BASSO; PILAR FERRARIS; HELGA BLANCO
Editorial:
EDITORIAL UNIVERSITARIA-UNSL
Referencias:
Lugar: SAN LUIS; Año: 2015 p. 252
ISSN:
9781111138363
Resumen:
CONTENTS1. RNA extraction using TRIZOL (Invitrogen) .................................................................................................. ……..12. Determining the Yield and Quality of Purified RNA ................................................................................ ……..23. PCR Primer Design .................................................................................................................................................... …...104. Nondenaturing Agarose Gel Electrophoresis of RNA.............................................................................. …...215. The Technique of In Situ Hybridization ......................................................................................................... …...256. Array-Based Nuclear Run-On Analysis ........................................................................................................... …...497. Chromatin Immunoprecipitation Assay as a Tool for Analyzing Transcription Factor Activity................................................................................................................................................................................................... …...628. CAGE (Cap Analysis of Gene Expression): A Protocol for the Detection of Promoter andTranscriptional Networks .......................................................................................................................................... ...…829. Native Chromatin Immunoprecipitation ....................................................................................................... …10210. Basic principles of real-time PCR .................................................................................................................... …12011. Optimization and Troubleshooting in PCR ................................................................................................ …15612. Reverse Transcriptase Assay (RT) ................................................................................................................. …16313. Detection of Small Non-coding RNAs ............................................................................................................ …16414. Quantitative Estimation of Multiple miRNAs and mRNAs from a Single Cell ......................... …17415. Human embryonic stem cell protocols ........................................................................................................ …18616. Microarray Protocols ............................................................................................................................................. …20517. Mobility Shift Assays.............................................................................................................................................. …22718. Usefulness of the Luciferase Reporter System to Test the Efficacy of siRNA .......................... …23619. RNA Immunoprecipitation to Determine RNA-Protein Associations In Vivo .......................... …243