5,5-dimethyl-1-pyrroline-N-oxide (DMPO) reduces hepatic neutrophilic inflammation and insulin resistance in obese mice

CARLA FLORENCIA GUZMAN ; INALEN CHACON; FLORENCIA SOLEDAD BARRERA,; JONATHAN M. RAMIREZ; LUCILA RIVEROS ; IVANA MORALES; SONIA ESTHER BARBERIS 5 , ; LUCAS SANTILLAN; DARIO C. RAMIREZ ; SANDRA GOMEZ-MEJIBA

Congreso; SOCIETY OF FREE RADICAL BIOLOGY AND MEDICINE; 2024

A chronic positive-energy balance causes low-grade systemic inflammation in diet-induced obesity(DIO) models. This causes the activation of the expression of inflammatory biomarkers, such asinducible nitric oxide synthase (iNOS), inflammatory cytokines, and adhesion molecules forneutrophils in tissue microvasculatures. In this study, our objective was to determine whether thenitrone 5,5-dimethyl-1-pyrroline N-oxide (DMPO) is capable of reducing hepatic neutrophilicinflammation, and systemic inflammation; and consequently improving insulin sensitivity in a DIOmouse model. To achieve this goal, we fed male C57 mice a control diet (CoD, rodent chow and tapwater) or a hypercaloric diet (ObD, 22% bovine fat + 10% fructose in drinking water) for 24 weeks.Food and water intake, body weight, and caloric intake were measured weekly. At the end of thedietary regime, serum IL-6, iNOS, nitrotyrosine formation (a marker of NO-induced oxidativestress), chlorotyrosine (a marker commonly used as a measure of neutrophilic inflammation), and