A dietary chronic-positive energy balance causes pulmonary neutrophilic inflammation in mice-

FLORENCIA BARRERA 1 , ; CARLA FLORENCIA GUZMAN 2 , ; INALEN CHACON; JONATHAN M. RAMIREZ ; , LUCILA RIVEROS ; IVANA MORALES; SONIA ESTHER BARBERIS; MARIA AIDA MATTAR DOMINGUEZ; LUCAS SANTILLAN; DARIO C. RAMIREZ ; SANDRA GOMEZ-MEJIBA

Congreso; SOCIETY OF FREE RADICAL BIOLOGY AND MEDICINE; 2024

A dietary chronic positive-energy balance causes low-grade systemic inflammation. Neutrophilicinflammation (NI) is the homing and activation of neutrophils in tissues with local release ofmyeloperoxidase (MPO) and generation of HOCl causing chlorinating stress, a marker of NI.Herein, we hypothesized that a dietary chronic positive energy balance can cause pulmonary NI. Totest this we fed, ad libitum, 2 groups of male C57 mice (n=32 each) for 24 weeks with either anobesogenic diet (ObD) consisting of rodent chow containing a 22% bovine fat (483 kCal/100g) and10% fructose in drinking water (3.9 Kcal/ml), or a control diet (CoD) containing rodent chow (329Kcal/100g) and tap drinking water. Weight gain, blood pressure, insulin resistance, and adiposityindex were higher in ObD compared to CoD. Plasma concentrations of total cholesterol,triglycerides, glutamic-oxaloacetic transaminase (GOT), and glutamic-pyruvic transaminase (GPT)were higher in ObD compared to CoD mice. Compared to the lungs of CoD, ObD mice had a highernumber of BALF neutrophils, content and activity of MPO, chlorotyrosine-modified proteins,inducible nitric oxide synthase, and IL-6. Interestingly, when instilled with bacteriallipopolysaccharide or dead Pseudomonas aeruginosa, ObD mice showed a higher MPO content andactivity, and chlorinated proteins than CoD mice. These data corroborate a higher content andactivation of neutrophils in the lung of ObD mice than in the CoD mice. A chronic dietary positiveenergy balance causes pulmonary neutrophilic inflammation i