A PRELIMINARY STUDY ABOUT THE CYTOTOXICITY AND PRO-INFLAMMATORY PROPERTIES OF NANODIAMONDS AND TIO2 NANOPARTICLES

KATHLEEN F. HINOJOSA VERA, ; CECILIA S. CÁCERES; MARIA A. MATTAR DOMÍNGUEZ; SANDRA E. GOMEZ MEJIBA; DARIO C. RAMIREZ; PATRICIA W. STEGE

Congreso; SAIC; 2024

Nanodiamonds (ND) are carbon nanoparticles (NPs) with uniqueoptical, chemical, and biological properties that make them applicablein medicine, pharmaceuticals, and personal care products.Therefore, before naming NDs as nanomaterials of biomedical interestmore studies are necessary to clarify the interaction with livingsystems, including cytotoxicity and pro-inflammatory outcomes.Macrophages are ubiquitous cells in all tissues and central cells inthe innate immune system. The measurement of the cytotoxic propertiesof nanomaterials on primary macrophage culture is the firststep in testing further biological effects. Furthermore, the inflammatorypriming of peritoneal macrophages can be directly studied bymeasuring the radical nitric oxide (•NO) production as nitrites in theculture medium. Therefore, in this study, we aim to test the cytotoxicand pro-inflammatory properties of TiO2 NPs and ND. To accomplishour goal, we isolated mouse peritoneal macrophages (PM)from peptone-elicited male 7-week-old BALB/c mice. Monolayers