PULMONARY NEUTROPHILIC INFLAMMATION LEADS TO INSULIN RESISTANCE IN A MOUSE MODEL OF DIET-INDUCED OBESITY

BARRERA FS; ,GUZMÁN CF; CHACON I DEL V; RAMIREZ JM; MATTAR DOMINGUEZ MA; RIVEROS L; MORALES; BARBIERIS S; RAMIREZ DC; GOMEZ MEJIBA SE

san luis

Congreso; SBC; 2024

008. PULMONARY NEUTROPHILIC INFLAMMATION LEADS TO INSULIN RESISTANCE IN A MOUSEMODEL OF DIET-INDUCED OBESITYBarrera FS1,Guzmán CF2, Chacon I del V2, Ramirez JM1,2, Mattar Dominguez MA3, Riveros L4, Morales I4,Barbieris S4,Ramirez DC2 & Gomez Mejiba SE11 Laboratorio de Nutrición y Terapéuticas Experimentales, CONICET-San Luis & Universidad Nacional de San Luis, San Luis, Argentina. 2Laboratorio de Medicina Experimental y Trasduccional, CONICET-San Luis & Universidad Nacional de San luis,San Luis,Argentina. 3 Laboratoriode Microbiología e Inmunología , CONICET-San Luis & Univerdidad Nacional de San Luis 4 Laboratorio de Bromatología, CONICET-San Luis &Universidad Nacional de San Luis, San Luis, Argentina. E-mail:fsoledadbarrera@gmail.comA chronic positive dietary energy balance causes low-grade systemic inflammation. Neutrophilic inflammation (NI) is the homing and activation ofneutrophils in tissues with local release of myeloperoxidase (MPO) and generation of HOCl causing chlorination stress, a marker of NI. In this work,we hypothesized that chronic positive dietary energy balance causes pulmonary NI. To test this, we fed, ad libitum, 2 groups of male C57 mice (n =16 each) for 24 weeks either an obesogenic diet (ObD) consisting of rodent chow containing 22% bovine fat (483 kcal/100 g) and 10% fructose in tapwater(3.9 kcal/ml), or a control diet (CoD) containing rodent chow (329 kcal/100 g) and tap-water. A group of CoD and ObD mice were intratracheallychallenged with vehicle (PBS), LPS (5 μg/mouse), or 107 heat-death Pseudomonas aeruginosa (Pa)/mouse. Finally, inducible nitric oxide synthase(iNOS), MPO, chlorotyrosine (Cl-Tyr)-modified proteins and IL-6 concentrations in lung homogenates were measured by (ELISA). Weight gain,blood pressure, insulin resistance, and adiposity index were higher (p