ROLE OF MYELOPEROXIDASE IN THE MODULATION OF INFLAMMATORY RESPONSES IN ANTERIOR PITUITARY IN RAT WITH POLYCYSTIC OVARY

MENDOZA GV; FIGUEROA MF; GÓMEZ MEJIBA SE; RAMIREZ DC; FORNERIS ML

Congreso; SBC; 2024

012 - ROLE OF MYELOPEROXIDASE IN THE MODULATION OF INFLAMMATORY RESPONSES INANTERIOR PITUITARY IN RAT WITH POLYCYSTIC OVARYMendoza GV1, Figueroa MF1, Gómez Mejiba SE2, Ramirez DC2, Forneris ML11 Facultad de Química, Bioquímica y Farmacia (UNSL). Ejército de los Andes 950. San Luis. 2Laboratorio de Medicina Experimental yTraduccional y Laboratorio de Terapéutica Experimental y Nutrición. IMIBIO-SL-CONICET-UNSL. Email: mlforneris@gmail.comPolycystic Ovary Syndrome (PCOS) is a heterogeneous functional endocrine-gynecology disorder that affects many women of childbearing age. It ischaracterized by hyperandrogenism and infertility. In addition to neuro-endocrine alterations, other mechanisms including oxidative stress and lowgradeinflammation are related to the pathogenesis of PCOS. Previously, we have demonstrated an immune-endocrine interaction, through of the effectof spleen macrophage secretion, in anterior pituitary (AP) of rats with polycystic ovary (PCO). These macrophages secretions from PCO rats increasedthe expression of pro-inflammatory interleukin (IL) genes, as IL1β and tumor necrosis factor (TNFα), and nitric oxide release (NO). Myeloperoxidase(MPO) is an enzyme released from immune cells as macrophages are involved in the pathological processes of diseases mainly through the oxidationof biomolecules, which promotes inflammation and oxidative stress. Thus, the aim of this work was to study, in AP from PCO-induced rat model, 1)the presence of MPO and its relationship with the expressions/release of inflammatory markers and 2) the effects of macrophage secretion on MPOcontent and its oxidant. The PCO condition was induced in adult female Holtzman rat with estradiol valerate (i.m injection 2 mg/rat) and 2 monthslater animals were sacrificed. PCO and Control pituitaries (PCO-AP and C-AP) were incubated with RPMI medium (basal value) and PCO macrophagesecretions (PCO-MϕS), for 3h in metabolic bath. In AP homogenates, MPO and its oxidants: nitrotyrosine (nitrosative stress) and chlorotyrosine (HOClinduced protein oxidation) and iNOS were determinate by ELISA assay. In culture supernatant, levels of TNFα and IL6 release were measured byELISA and their genic expressions were analyzed by RT-PCR. In basal condition: MPO, nitrotyrosine and chlorotyrosine concentration were increasedin the PCO-AP group compared with C-AP (p