INVESTIGADORES
GARRIZ andres
congresos y reuniones científicas
Título:
Differential roles for polyamines in the oxidative stress response of Pseudomonas syringae
Autor/es:
SOLMI LEANDRO; FRANCO R. ROSSI; FERNANDO M. ROMERO; OA RUIZ; BACH-PAGES M; PRESTON G; GÁRRIZ ANDRES
Lugar:
Southampton
Reunión:
Simposio; 6TH YOUNG MICROBIOLOGIST SYMPOSIUM ON MICROBE SIGNALLING, ORGANISATION AND PATHOGENESIS; 2020
Institución organizadora:
University of Southampton
Resumen:
Putrescine and spermidine are the main polyamines (PAs) found in bacteria. These compoundscomprehend a family of small aliphatic molecules implicated in protein synthesis and regulation,DNA integrity and stress resistance. The contribution of PAs to oxidative stress tolerance inhuman pathogens such as Escherichia coli and Pseudomonas aeruginosa has been clearlydocumented, but their functions in the stress response activated by other types of bacteria havenot been poorly explored. In this study, we unveiled an important role for PA homeostasis duringthe oxidative stress response in the phytopathogenic bacteria Pseudomonas syringae pv. tomatoDC3000. WT cells exposed to sublethal concentrations of hydrogen peroxide induced thesecretion of PAs but maintained their intracellular concentrations in similar levels as those foundin non-treated cells, suggesting a role for extracellular PAs. In turn, pre-treatment withspermidine enhanced stress susceptibility, whereas putrescine-treatment had no effect onstress tolerance. In agreement with this, a double mutant in the speA and speC genes showingundetectable levels of putrescine showed higher stress susceptibility, but disruption of thespermidine biosynthetic gene speE enhanced stress tolerance. These results demonstratecontrasting roles played by PAs in the response to oxidative stress in this bacterial species.