INVESTIGADORES
COMBINA Mariana
artículos
Título:
Biocontrol of fungi isolated from sour rot infected table grapes by Saccharomyces and other yeast species
Autor/es:
NALLY M.C.; PESCE M. V.; MATURANO Y.P.; TORO M.E.; COMBINA M.; CASTELLANOS DE FIGUEROA L.I.; VAZQUEZ F.
Revista:
POSTHARVEST BIOLOGY AND TECHNOLOGY
Editorial:
ELSEVIER SCIENCE BV
Referencias:
Lugar: Amsterdam; Año: 2013 vol. 86 p. 456 - 462
ISSN:
0925-5214
Resumen:
Abstract: Sour rot is an important disease of grapes caused by an etiologic complex of microorganisms in which filamentous fungi play a key role. Yeasts are used as biocontrol of pathogenic filamentous fungi in fruits. The major objective of the present work was to assess the in vivo effect of viticultural yeasts on phytopathogenic fungi involved in grape sour rot. Yeasts that were found to be effective in vivo against the fungi were assayed for their possible pathogenicity in humans: growth at 42 °C, pseudohyphal formation, adhesion, and phospholipase and protease activity. A total of 234 yeasts belonging to 14 genera were assayed against the following pathogens: Aspergillus caelatus, Aspergillus carbonarius, Aspergillus terreus, Aspergillus versicolor, Fusarium oxysporum, Penicillium comune, Rhizopus stolonifer and Ulocladium sp. Forty-three (16 Saccharomyces and 27 non-Saccharomyces) showed antagonistic properties against some of the fungi assayed in grapes at 25 °C. Yeast isolates determined as biocontrol agents under in vivo conditions were isolated from fermenting musts (35), viticultural soils (6) and grape berries (2). Twenty biocontrol agents did not show phenotypical characteristics associated with pathogenicity in humans. This is the first study reporting on the antagonistic property of viticultural yeasts against 8 hytopathogenic fungi isolated from sour rot-damaged grapes. This fact makes our results even more relevant.