The karyotype of Alouatta pigra (Primates: Platyrrhini): mitotic and meoitic analyses

STEINBERG, ELIANA R.; CORTEZ-ORTIZ, LILIANA; NIEVES, MARIELA; BOLZÁN, ALEJANDRO; GARCIA-ORDUÑA, FRANCISCO; HERMIDA-LAGUNES, JAVIER; CANALES ESPINOZA, DOMINGO; MUDRY, MARTA D.

CYTOGENETICS AND CELL GENETICS

Karger

Año: 2008

0301-0171

We describe for the first time the karyotype of the black howler monkey, Alouatta pigra. Conventional staining, G and C banding, and fluorescence in situ hybridization (FISH) with a peptide nucleic acid (PNA) pantelomeric probe were conducted. Eight free ranging individuals, four male and four female adult specimens, within the natural distribution of the species presented 2N=58. Mitotic analyses showed an autosomal complement composed by 6 submetacentric chromosome pairs, 3 metacentric, and 19 acrocentric for females and 6 submetacentric, 3 metacentric, and 18 acrocentric for males. Meiotic analyses in males revealed 27 autosomal bivalents and a sexual quadrivalent composed by a submetacentric X1 and acrocentric X2, Y1, and Y2. The G banded karyotype allowed us to identify pair #17 as the autosomal pair involved in the rearrangement and the morphology of the sexual quadrivalent components. C-banding technique in Metaphase I corroborated the structure of the sexual quadrivalent showing four centromeres C+. FISH analysis showed telomeric signals at the terminal regions of all chromosomes. No interstitial signals were detected. DNA sequence data were in accordance with those previously published for this species. us to identify pair #17 as the autosomal pair involved in the rearrangement and the morphology of the sexual quadrivalent components. C-banding technique in Metaphase I corroborated the structure of the sexual quadrivalent showing four centromeres C+. FISH analysis showed telomeric signals at the terminal regions of all chromosomes. No interstitial signals were detected. DNA sequence data were in accordance with those previously published for this species. with a peptide nucleic acid (PNA) pantelomeric probe were conducted. Eight free ranging individuals, four male and four female adult specimens, within the natural distribution of the species presented 2N=58. Mitotic analyses showed an autosomal complement composed by 6 submetacentric chromosome pairs, 3 metacentric, and 19 acrocentric for females and 6 submetacentric, 3 metacentric, and 18 acrocentric for males. Meiotic analyses in males revealed 27 autosomal bivalents and a sexual quadrivalent composed by a submetacentric X1 and acrocentric X2, Y1, and Y2. The G banded karyotype allowed us to identify pair #17 as the autosomal pair involved in the rearrangement and the morphology of the sexual quadrivalent components. C-banding technique in Metaphase I corroborated the structure of the sexual quadrivalent showing four centromeres C+. FISH analysis showed telomeric signals at the terminal regions of all chromosomes. No interstitial signals were detected. DNA sequence data were in accordance with those previously published for this species. us to identify pair #17 as the autosomal pair involved in the rearrangement and the morphology of the sexual quadrivalent components. C-banding technique in Metaphase I corroborated the structure of the sexual quadrivalent showing four centromeres C+. FISH analysis showed telomeric signals at the terminal regions of all chromosomes. No interstitial signals were detected. DNA sequence data were in accordance with those previously published for this species. Conventional staining, G and C banding, and fluorescence in situ hybridization (FISH) with a peptide nucleic acid (PNA) pantelomeric probe were conducted. Eight free ranging individuals, four male and four female adult specimens, within the natural distribution of the species presented 2N=58. Mitotic analyses showed an autosomal complement composed by 6 submetacentric chromosome pairs, 3 metacentric, and 19 acrocentric for females and 6 submetacentric, 3 metacentric, and 18 acrocentric for males. Meiotic analyses in males revealed 27 autosomal bivalents and a sexual quadrivalent composed by a submetacentric X1 and acrocentric X2, Y1, and Y2. The G banded karyotype allowed us to identify pair #17 as the autosomal pair involved in the rearrangement and the morphology of the sexual quadrivalent components. C-banding technique in Metaphase I corroborated the structure of the sexual quadrivalent showing four centromeres C+. FISH analysis showed telomeric signals at the terminal regions of all chromosomes. No interstitial signals were detected. DNA sequence data were in accordance with those previously published for this species. us to identify pair #17 as the autosomal pair involved in the rearrangement and the morphology of the sexual quadrivalent components. C-banding technique in Metaphase I corroborated the structure of the sexual quadrivalent showing four centromeres C+. FISH analysis showed telomeric signals at the terminal regions of all chromosomes. No interstitial signals were detected. DNA sequence data were in accordance with those previously published for this species. with a peptide nucleic acid (PNA) pantelomeric probe were conducted. Eight free ranging individuals, four male and four female adult specimens, within the natural distribution of the species presented 2N=58. Mitotic analyses showed an autosomal complement composed by 6 submetacentric chromosome pairs, 3 metacentric, and 19 acrocentric for females and 6 submetacentric, 3 metacentric, and 18 acrocentric for males. Meiotic analyses in males revealed 27 autosomal bivalents and a sexual quadrivalent composed by a submetacentric X1 and acrocentric X2, Y1, and Y2. The G banded karyotype allowed us to identify pair #17 as the autosomal pair involved in the rearrangement and the morphology of the sexual quadrivalent components. C-banding technique in Metaphase I corroborated the structure of the sexual quadrivalent showing four centromeres C+. FISH analysis showed telomeric signals at the terminal regions of all chromosomes. No interstitial signals were detected. DNA sequence data were in accordance with those previously published for this species. us to identify pair #17 as the autosomal pair involved in the rearrangement and the morphology of the sexual quadrivalent components. C-banding technique in Metaphase I corroborated the structure of the sexual quadrivalent showing four centromeres C+. FISH analysis showed telomeric signals at the terminal regions of all chromosomes. No interstitial signals were detected. DNA sequence data were in accordance with those previously published for this species. Alouatta pigra. Conventional staining, G and C banding, and fluorescence in situ hybridization (FISH) with a peptide nucleic acid (PNA) pantelomeric probe were conducted. Eight free ranging individuals, four male and four female adult specimens, within the natural distribution of the species presented 2N=58. Mitotic analyses showed an autosomal complement composed by 6 submetacentric chromosome pairs, 3 metacentric, and 19 acrocentric for females and 6 submetacentric, 3 metacentric, and 18 acrocentric for males. Meiotic analyses in males revealed 27 autosomal bivalents and a sexual quadrivalent composed by a submetacentric X1 and acrocentric X2, Y1, and Y2. The G banded karyotype allowed us to identify pair #17 as the autosomal pair involved in the rearrangement and the morphology of the sexual quadrivalent components. C-banding technique in Metaphase I corroborated the structure of the sexual quadrivalent showing four centromeres C+. FISH analysis showed telomeric signals at the terminal regions of all chromosomes. No interstitial signals were detected. DNA sequence data were in accordance with those previously published for this species. us to identify pair #17 as the autosomal pair involved in the rearrangement and the morphology of the sexual quadrivalent components. C-banding technique in Metaphase I corroborated the structure of the sexual quadrivalent showing four centromeres C+. FISH analysis showed telomeric signals at the terminal regions of all chromosomes. No interstitial signals were detected. DNA sequence data were in accordance with those previously published for this species. with a peptide nucleic acid (PNA) pantelomeric probe were conducted. Eight free ranging individuals, four male and four female adult specimens, within the natural distribution of the species presented 2N=58. Mitotic analyses showed an autosomal complement composed by 6 submetacentric chromosome pairs, 3 metacentric, and 19 acrocentric for females and 6 submetacentric, 3 metacentric, and 18 acrocentric for males. Meiotic analyses in males revealed 27 autosomal bivalents and a sexual quadrivalent composed by a submetacentric X1 and acrocentric X2, Y1, and Y2. The G banded karyotype allowed us to identify pair #17 as the autosomal pair involved in the rearrangement and the morphology of the sexual quadrivalent components. C-banding technique in Metaphase I corroborated the structure of the sexual quadrivalent showing four centromeres C+. FISH analysis showed telomeric signals at the terminal regions of all chromosomes. No interstitial signals were detected. DNA sequence data were in accordance with those previously published for this species. us to identify pair #17 as the autosomal pair involved in the rearrangement and the morphology of the sexual quadrivalent components. C-banding technique in Metaphase I corroborated the structure of the sexual quadrivalent showing four centromeres C+. FISH analysis showed telomeric signals at the terminal regions of all chromosomes. No interstitial signals were detected. DNA sequence data were in accordance with those previously published for this species. in situ hybridization (FISH) with a peptide nucleic acid (PNA) pantelomeric probe were conducted. Eight free ranging individuals, four male and four female adult specimens, within the natural distribution of the species presented 2N=58. Mitotic analyses showed an autosomal complement composed by 6 submetacentric chromosome pairs, 3 metacentric, and 19 acrocentric for females and 6 submetacentric, 3 metacentric, and 18 acrocentric for males. Meiotic analyses in males revealed 27 autosomal bivalents and a sexual quadrivalent composed by a submetacentric X1 and acrocentric X2, Y1, and Y2. The G banded karyotype allowed us to identify pair #17 as the autosomal pair involved in the rearrangement and the morphology of the sexual quadrivalent components. C-banding technique in Metaphase I corroborated the structure of the sexual quadrivalent showing four centromeres C+. FISH analysis showed telomeric signals at the terminal regions of all chromosomes. No interstitial signals were detected. DNA sequence data were in accordance with those previously published for this species. us to identify pair #17 as the autosomal pair involved in the rearrangement and the morphology of the sexual quadrivalent components. C-banding technique in Metaphase I corroborated the structure of the sexual quadrivalent showing four centromeres C+. FISH analysis showed telomeric signals at the terminal regions of all chromosomes. No interstitial signals were detected. DNA sequence data were in accordance with those previously published for this species. 1 and acrocentric X2, Y1, and Y2. The G banded karyotype allowed us to identify pair #17 as the autosomal pair involved in the rearrangement and the morphology of the sexual quadrivalent components. C-banding technique in Metaphase I corroborated the structure of the sexual quadrivalent showing four centromeres C+. FISH analysis showed telomeric signals at the terminal regions of all chromosomes. No interstitial signals were detected. DNA sequence data were in accordance with those previously published for this species.