Adapting the buccal micronucleus cytome for use in dogs

VALERIA LENTINI; DANIELA M FERRÉ; NORA B GORLA

Mendoza

Congreso; XXXI Reunión Científica Anual de la Sociedad de Biología de Cuyo; 2013

The Buccal Micronucleus Cytome (BMCyt) assay has been used to successfully quantify genetic damage in humans caused by a variety of genotoxic agents.The objective of this study was to adapt the BMCyt assay for use on dogs and to identify the type and frequencies ofcommon nuclear abnormalities detected in dogscells for comparison to those detected in human studies.Pet dogs may be particularly valuable sentinels to environmental health because they share the human places and respond to toxic insults in ways analogous to humans. Buccal cells were collected from 13 purebred dogs, 20 days old, 5 males and 7 females.After fixation and Giemsa stained, 850 cells per animal were analyzed. Surprisingly micronuclei (MN) were not detected in any sample. The frequency of binucleated cells were 4,6±3,0 and lobed nuclei were 5,8±3,1/ 850 analyzed cells. The absence of MN may be due the genetically not damaged condition as newborn animals or because the specie is particularly efficient in spleen micronucleated cells remotion. MN must to be searched in dogs intentionally exposed to genotoxic insult. Lobed nuclei is a category not present in human cells, and its significance must be investigated. Examination of MN frequencies in the buccal cells of dogs has not previously been attempted.