INVESTIGADORES
FRANK fernanda Maria
artículos
Título:
Redirection of the immune response to the functional catalytic domain of cruzipain improves protective immunity against Trypanosoma cruzi infection
Autor/es:
CAZORLA SI; FRANK FM; BECKER PD; ARNAIZ M; MIRKIN GS; CORRAL RS; GUZMAN CA; MALCHIODI EL
Revista:
JOURNAL OF INFECTIOUS DISEASES
Editorial:
University of Chicago Press
Referencias:
Año: 2010 vol. 201
ISSN:
0022-1899
Resumen:
Despite the strong responses elicited after natural infection or vaccination, Trypanosoma cruzi survival suggests that immune responses are insufficient or inherently inadequate. T. cruzi contains a major cystein proteinase, cruzipain, which has a catalytic N-terminal domain, and a C-terminal extension. Immunizations employing recombinant cruzipain (rCz) or its N- and C-terminal domains allowed dissecting the ability of Cz to circumvent responses against the catalytic domain. This phenomenon is not a property of the parasite, but of Cz itself, since rCz triggers a similar effect to Cz during natural or experimental infection. Cz is not the only antigen having a highly immunogenic region of unknown function which somehow protects an essential domain for parasite survival. However, our studies show that this can be reverted by using the N-terminal domain as tailored immunogen able to redirect host responses to provide enhanced protection.