INVESTIGADORES
MARTINEZ eric Javier
artículos
Título:
Sequence characterization, in silico mapping and cytosine methylation analysis of markers linked to apospory in Paspalum notatum.
Autor/es:
PODIO, MARICEL; RODRIGUEZ, MARÍA P.; FELITTI, SILVINA A.; STEIN, JULIANA; MARTÍNEZ, ERIC J.; SIENA, LORENA A.; QUARIN, CAMILO L.; PESSINO, SILVINA C.; ORTIZ, JUAN P. A.
Revista:
GENETICS AND MOLECULAR BIOLOGY
Editorial:
SOC BRASIL GENETICA
Referencias:
Año: 2012 vol. 35 p. 827 - 837
ISSN:
1415-4757
Resumen:
In previous studies we reported the identification of several AFLP, RAPD and RFLP molecular markers linked to apospory in Paspalum notatum. The objective of this work was to sequence these markers, obtain their flanking regions by chromosome walking, and perform an in silico mapping analysis in rice and maize. Moreover, methylation sensitive RFLP experiments were carried out to investigate the methylation status of two apospory-related sequences. Fourteen molecular markers were analyzed. Several protein-coding sequences were identified. Copy number estimations and RFLP linkage analysis showed that sequence PnMAI3 displayed 2-4 copies per genome and linkage to apospory. Extension of this marker by chromosome walking revealed an additional protein-coding sequence mapping in silico at the apospory-syntenic regions of rice and maize. Through the global sequencing procedure, ~ 5 kb corresponding to different markers were characterized. Sequence information allowed a more refined synteny analysis with model species. Results indicate synteny with segments of chromosomes 2 and 12 of rice, as well as 3 and 5 of maize. Two apomixis-associated loci were tested in methylation-sensitive RFLP experiments on genomic DNA extracted from leaves. Although both target sequences were methylated, no methylation polymorphisms associated with the mode of reproduction were detected.