INVESTIGADORES
GARCIA Veronica Edith
congresos y reuniones científicas
Título:
ROLE OF PGE2 ON NEUTROPHILS DURING HUMAN TUBERCULOSIS.
Autor/es:
CANDELA MARTIN; JOAQUIN PELLEGRINI; MARIA PAULA MORELLI; N. TATEOSIAN; AMIANO NICOLAS; A LORENA CIALLELA,; DOMINGO J. PALMERO; GARCÍA, VERÓNICA EDITH
Reunión:
Simposio; Keystone Symposium,"Tuberculosis: Science Aimed at Ending the Epidemic"; 2020
Resumen:
Tuberculosis (TB) is one of the top 10 causes of death worldwide. Prostaglandin E2 (PGE2), an active lipid compound derived from arachidonic acid, regulates different stages of the response of the host during several pathologies such as chronic infections or cancer. Interestingly, manipulation of PGE2 levels was proposed as an approach for countering the Type I IFN signature of TB 1, although very limited information about this pathway in tuberculosis patients is available. Therefore, the aim of the present work was to investigate the role of this compound during human TB. Neutrophils were obtained from heparinized peripheral blood from healthy donors (HD) and tuberculosis patients (TB) by Ficoll-Hypaque centrifugation and Dextran sedimentation. Cells were cultured (2x106 cells/ml) with a Mycobacterium tuberculosis lysate (Mtb-Ag, 10μg/ml) with/without PGE2. Then, we evaluated the role of this lipidic mediator during the human immune response against Mtb-Ag by using flow cytometry and confocal microscopy assays. P-values