NEUTROPHIL AUTOPHAGY DURIING HUMAN ACTIVE TUBERCULOSIS IS MODULATED BY SLAMF1.

JOAQUIN PELLEGRINI; FLORENCIA SABBIONE; MARIA PAULA MORELLI; CANDELA MARTIN; N. TATEOSIAN; AMIANO NICOLAS; DOMINGO J. PALMERO; MARIA ISABEL COLOMBO; ANALIA TREVANI; GARCÍA V.E

Congreso; LXVII REUNIÓN ANUAL DE LA SOCIEDAD ARGENTINA DE INMUNOLOGÍA; 2019

NEUTROPHIL AUTOPHAGY DURIING HUMAN ACTIVE TUBERCULOSIS IS MODULATED BY SLAMF1.BACKGROUND: Neutrophils infected with Mycobacterium tuberculosis (Mtb) predominate in patient´s lungs. Neutrophilic infiltration represents a characteristic feature of severe tuberculosis (TB) but neutrophil biology during TB remains poorly understood. Autophagy is crucial for several neutrophil functions and can be modulated by different mediators. The costimulator SLAMF1 acts as a microbial sensor in macrophages but its expression in human neutrophils has not been evaluated. METHODS: We investigated whether SLAMF1 participates in neutrophil autophagy against Mtb. Neutrophils were isolated from heparinized blood from TB patients and healthy donors and cultured (2x106 cells/ml) with Mtb-Ag (10μg/ml) ± SLAMF1 mAb (10μg/ml) during 2 h. Some experiments were performed in the presence of a NADPH-oxidase inhibitor. SLAMF1 and autophagy levels were analyzed by flow cytometry and confocal microscopy. P values of