EFFECT OF IL-1β ON THE AUTOPHAGY PROCESS IN MONOCYTES FROM TUBERCULOSIS PATIENTS

CANDELA MARTIN; MORELLI, MARÍA PAULA; NANCY L. TATEOSIÁN; AMIANO, NICOLÁS O; LUCIA DONOLLI; A LORENA CIALLELA,; , ROSA M. MUSELLA,; ADRIANA RODRIGUEZ MIERES; RODRIGO FAILDE; GRACIELA CRAGNOLINI CASADO; DOMINGO J. PALMERO; VERONICA E GARCIA; GARCÍA, VERÓNICA

Congreso; LXXI Argentinean Society for Immunology Meeting. San Luis, Argentina. 2023.; 2023

INTRODUCTION: Autophagy, an essential process that maintains cell homeostasis, is also an important protective mechanism against Mycobacterium tuberculosis (Mtb) infection and other intracellular pathogens. It has been demonstrated that the induction of autophagy suppressed intracellular survival of Mtb. Interleukin 1β (IL-1β) is a key mediator of the inflammatory response, essential for the host-response and resistance to pathogens. We previously reported that the cytokines IFNγ and IL-17A augmented autophagy in Mtb-infected monocytes from tuberculosis (TB) patients, according to their immunological status of the human host. Here we investigated the role of IL-1β on the autophagy process in two groups of TB patients. Our ultimate goal is to elucidate if this cytokine could play a role in a host directed therapy.METHODS: TB patients were classified as low and high responders (LR and HR, respectively) according to their immune responses to sonicated Mtb (Mtb-Ag). Control individuals were healthy donors (HD) from the community. Monocytes were obtained from heparinized peripheral blood and cultured (2×106 cells/ml) with an Mtb lysate (Mtb-Ag, 10μg/ml) ± IL-1β (10ng/ml). To evaluate the effect of IL-1β on bacterial growth, monocytes were infected with H37Rv Mtb strain (MOI: 10). After 2h of infection, the medium was replaced and cells were cultured ± IL-1β (10 ng/ml) for 96h. Cells were then washed and lysed to assay mycobacterial colony-forming units (CFU). Flow cytometry was used to evaluate autophagy levels by determining LC3 (the main marker of the autophagy process). P-values