CYTOKINE MODULATION OF AUTOPHAGY IN NEUTROPHILS FROM TUBERCULOSIS PATIENTS WITH DIFFERENT SEVERITY OF THE DISEASE.

MARÍA PAULA MORELLI ; CANDELA MARTIN; LUCIA DONNOLI ; CAMILA MARTINENA; NANCY TATEOSIAN, ; NICOLAS OSCAR AMIANO; LORENA CIALLELLA; ROSA MARÍA MUSELLA; ADRIANA RODRÍGUEZ MIERES; GRACIELA M. GRAGNOLINI DE CASADO2,; DOMINGO PALMERO; VERÓNICA EDITH GARCÍA

Congreso; Reunión conjunta SAIC-SAI-AAFE-NANOMED.AR 2022; 2022

INTRODUCTION: Human immune responses against Mycobacterium tuberculosis (Mtb) involve different cell types and immune mediators. Underlying tuberculosis (TB) clinical manifestations, individual immunological profiles can be identified in patients according to their immune responses during infection. Mtb infected neutrophils predominate in patient ́s lungs but their functions remain poorly understood. Here we investigated if the two interrelated cytokines IFNα and IL-1β regulate autophagy in patients with different immune responses to Mtb.METHODS: TB patients were classified as low and high responders (LR and HR, respectively) according to their immune responses to sonicated Mtb (Mtb-Ag). Neutrophils were isolated from heparinized blood and suspended at 2 × 106 cell/mL in supplemented media. Cells were then stimulated with Mtb-Ag (10 μg/mL) ± IL-1β (10 μg/mL) or INFα (5 μg/mL) and LC3 levels were determined by flow cytometry and confocal microscopy. P values of