Neutrophil autophagy during human active tuberculosis is modulated by SLAMF1

PELLEGRINI, JOAQUÍN M.; FLORENCIA SABBIONE; MARIA PAULA MORELLI; NANCY L. TATEOSIÁN; CASTELLO, FLORENCIA A.; AMIANO, NICOLÁS O.; DOMINGO J. PALMERO; ALBERTO LEVI; CIALLELLA, LORENA M.; MARIA ISABEL COLOMBO; ANALIA TREVANI; GARCÍA V.E

AUTOPHAGY

LANDES BIOSCIENCE

Lugar: Austin, Texas; Año: 2020

1554-8627

Neutrophils infected with Mycobacterium tuberculosis (Mtb) predominate in tuberculosispatients? lungs. Neutrophils phagocytose the pathogen, but its elimination is controversial.Macroautophagy/autophagy, a crucial mechanism for several neutrophils? functions, can bemodulated by immunological mediators. The costimulatory molecule SLAMF1 can act as amicrobial sensor in macrophages being also able to interact with autophagy-related proteins.Here, we demonstrate for the first time that human neutrophils express SLAMF1 upon Mtbstimulation.Furthermore, SLAMF1 was found colocalizing with LC3B+ vesicles, andactivation of SLAMF1 increased neutrophil autophagy induced by Mtb. Finally, tuberculosispatients´ neutrophils displayed reduced levels of SLAMF1 and lower levels of autophagyagainst Mtb as compared to healthy controls. Altogether, these results indicate that SLAMF1participates in neutrophil autophagy during active tuberculosis.