Functional evaluation of ToxA promoter in Trichoderma reesei Rut-C30

ORTIZ GE; ALBERTÓ E.; BLASCO M.

Biotechnology an Indian Journal

Trade Science Inc

Año: 2016 vol. 12 p. 39 - 44

0974-7435

In order to expand the collection of promoters studied in Trichodermareesei Rut-C30, a functional study of the ToxA promoter was carried out byanalysis of GFP expression in T. reesei Rut-C30. For this purpose, thebinary pCBCT expression vector was employed to transform T. reesei Rut-C30. The transformants obtained were evaluated by means of fluorescencemicroscopy, fluorometry, dot blot and Western blot analysis. The low levelsof cytoplasmic GFP protein in fungal hyphae suggest that ToxA promoterworks as a weak constitutive promoter which can drive successfully theexpression of heterologous proteins in T. reesei Rut-C30