ALBERTO edgardo Omar
Effect of co-cultivation of two Pleurotus species on lignocellulolytic enzyme production and mushroom fructification.
CARBAJAL, N. I.; LEVIN L.; ALBERTÓ E.; LECHNER B. E.
INTERNATIONAL BIODETERIORATION & BIODEGRADATION
ELSEVIER SCI LTD
Año: 2012 vol. 66 p. 71 - 76
Co-cultivation of mushroom species produce changes on fungal enzyme production and fructiﬁcation that were not in-depth studied hitherto. This work was conducted with the aim of studying how the interactions between Pleurotus ostreatus and Pleurotus citrinopileatus in a dual culture affect the ligno-cellulolytic enzymes production, inﬂuence yield values and modify the morphological properties of basidiomata obtained. With this purpose monocultures of both species in a straw-based substrate were investigated, as well as dual cultures with 3 different spatial patterns (one fungus in the upper portion of the bag and the second in the bottom, the opposite spatial pattern, and a mix of spawn from both, scattered in each bag). Co-cultivation did not increase basidiomata production, moreover, it inhibited carpophore development of P. ostreatus in the mix, and decreased basidiomata stems length signiﬁ-cantly. Biological efﬁciencies achieved in monocultures of P. ostreatus and P. citrinopileatus were 95.3%and 67.6% respectively. Different spatial patterns affected not only enzyme production but also mycelial yield. Cellulolytic and xylanolytic activities detected increased signiﬁcantly when P. ostreatus was inocOulated in the upper portion of the bag and P. citrinopileatus in the bottom part, but co-cultivation did not stimulate ligninolytic enzyme production. The highest laccase (8.2 U g1 ) and Mn-peroxidase (7.5 U g1 ) activities were detected in monocultures of P. ostreatus, in coincidence with maximum lignin degradation (28%).