INVESTIGADORES
CARGNELUTTI ethelina
congresos y reuniones científicas
Título:
Cytokine profile involved on Th17 differentiation in TNFRp55-/- mice with Yersinia induced-reactive arthritis
Autor/es:
CARGNELUTTI E.; ELIÇABE RJ; DI GENARO MS
Lugar:
Mendoza
Reunión:
Congreso; XXVI Reunión Científica Anual Sociedad de Biología de Cuyo - I Reunión Dirección Investigación, Ciencia y Técnica Ministerio de Salud ? Gobierno de Mendoza; 2008
Institución organizadora:
Sociedad de Biología de Cuyo/ Dirección Investigación, Ciencia y Técnica Ministerio de Salud ? Gobierno de Mendoza
Resumen:
We demonstrated that TNFRp55 deficient mice develop reactive arthritis (ReA) after oral Yersinia enterocolitica O:3 infection. The objective was to compare the cytokine profile involved in T helper (Th) 17 differentiation in mucosal and articular regional lymph nodes of infected C57BL/6 wild-type (WT) and TNFRp55-/- (KO) mice. The mice were orally infected with 1-6 x 108 colony-forming units (CFU) of Y. enterocolitica O:3. At days 7, 14 and 21 after infection, the mice were sacrificed and mesenteric (MLN) and inguinal (ILN) lymph nodes were aseptically removed. Organ homogenates were prepared and clarified by centrifugation. The supernatants were used for cytokine determinations. Interleukin (IL)-17, tumor growth factor (TGF)-beta and IL-6 levels were measured by ELISA. At day 7, we found higher levels of IL-17 in KO than WT mice in MLN (p