INVESTIGADORES
VOTA daiana Marina
congresos y reuniones científicas
Título:
Trophoblast giant cells profile and immune microenvironment are modulated by endogenous VIP in murine implantation sites
Autor/es:
V HAUK; G CALO; L GALLINO; D VOTA; R MEISS; R RAMHORST; J WASCHEK; C PÉREZ LEIRÓS
Lugar:
Mar del Plata
Reunión:
Congreso; Reunión Anual SAIC; 2016
Resumen:
Adequate trophoblast differentiation in an invasive phenotype is vital to ensure proper placentation. Among the factors that may facilitate trophoblast invasion are matrix metalloproteinases (MMP). Besides, trophoblast cells modulate leukocyte recruitment and function to maintain an anti-inflammatory and immunosuppressant microenvironment. Vasoactive intestinal peptide (VIP) is a pleiotropic peptide with immunomodulatory effects through its action on VPAC1 and VPAC2 receptors. Using different murine models we have shown that VIP favors a suitable anti-inflammatory and immunosuppressant profile for placentation and fetal growth. Moreover, we have found that VIP deficient pregnant mice have reduced litter size, increase the period inter-gestations and present an asymmetric distribution of implantation sites.Here we studied the relevance of endogenous VIP at the early maternal-placental interface and its impact on the local expression of immune and trophoblast functional markers in VIP KO mice.We isolated implantation sites from C57BL/6 WT female mated to VIP-/- or WT males at day 8,5 of pregnancy and analyzed immune markers by RTqPCR, the percentage of Treg cells by flow cytometry or used laser capture microdissection (LCM) to isolate trophoblast giant cells (TGC) for the analysis of VPACs expression, MMP9 and TGC markers by RTqPCR.Implantation site explants from WT mothers crossed with VIP -/- males presented a reduced percentage of Foxp3+ Treg cells within CD4+ cells (X±SEM: 10,3±1,7% WTxWT vs 5,2 ±1,3%WTxKO) and decreased IL-10 expression compared with WTxWT matings (P