VIP ENHANCES APOPTOTIC CELLs PHAGOCYTOSIS BY MONOCYTE/MACROPHAGES IN AN IN VITRO MODEL OF IMMUNE-TROPHOBLAST INTERACTION

PAPARINI DANIEL; GRASSO ESTEBAN; CALO GUILLERMINA; VOTA DAIANA; RAMHORST ROSANNA; PÉREZ LEIRÓS

Mar del Plata

Congreso; VI SLIMP-LASRI annual congress; 2015

Homeostasis maintenance at the early maternal-fetal interface requires the clearance of apoptotic cells by macrophages bearing an immunosuppressant profile. VIP is a pleiotropic polypeptide synthesized by trophoblast cells that promotes an anti-inflammatory profile in monocytes (Mo) and macrophages (Ma). However, its role in the modulation of macrophages at early pregnancy has not been elucidated yet. Objective: To explore VIP effect on the trophoblast-monocyte/macrophage interaction with special focus on Mo/Ma migration, phenotype and phagocytosis of apoptotic cells. Methods: Mo were purified by Percoll from blood mononuclear cells of healthy women and cultured with conditioned media (CM) from a first trimester human cytotrophoblast cell line (Swan-71, Tb) in the presence/ absence of VIP (10-100 nM). Apoptosis was induced in Tb with camptothecin. CFSE-labeled apoptotic cell phagocytosis by CD14+ cells and migration through 5 mm pore transwells was determined by FACS. Expression of CD39, IL-10, IL-12, TNF-a and adhesion molecules was also evaluated by FACS. Thrombospondin expression was determined by RTPCR. Results: CM from Tb cells treated for 18 h with VIP (CM-VIP) increased CD14+ cell migration to a higher extent than CM alone (p