METABOLIC PATHWAYS IN NEUTROPHILS IN A MODEL OF EARLY MATERNALPLACENTAL INTERACTION

CALO, GUILLERMINA; VOTA, DAIANA; SABBIONE, FLORENCIA; MERECH, FÁTIMA; HAUK, VANESA; RAMHORST, ROSANNA; TREVANI, ANALÍA; PÉREZ LEIRÓS, CLAUDIA

Congreso; Reunión Anual de la Sociedad Argentina de Inmunología; 2022

Trophoblast cells (Tb) interact with maternal immune cells at placentation favoring an anti-inflammatory microenvironment requiredfor fetal growth. Circulating neutrophils (PMN) appear activatedduring pregnancy and even more in pregnancies complicated bypreeclampsia. Metabolic regulation underlies the functional proflingof immune cells in a number of settings but immunometabolic reprogramming in the context of pregnancy is still enigmatic.We have shown that conditioned media (CM) from human frst trimester Tb (Swan-71 cell line) inhibit PMA-induced neutrophil extracellular trap formation, promote PMN apoptosis and induce anangiogenic profle on PMN.The aim of this work was to explore the effect of trophoblastcell-derived factors in neutrophils? functional and metabolic profle.Neutrophils from healthy donors were cultured with Swan-71 conditioned media (CM)-2% FCS. PMN activation profles were assessedby RT‐qPCR and flow cytometry. Treg modulation was exploredco-culturing PMN with autologous mononuclear cells and CD4,FOXP3 staining. Glucose uptake and intracellular lipid accumulationwere determined by flow cytometry using the glucose fluorescentanalog 2-NBDG or BODIPY 493/503, respectively.PMN pre-incubated with CM increased the frequency of doublestained CD4+ FOXP3+ cells in co-cultures, compared to 2% serummedia (basal) (% X±SE Basal 3.85±0.64 CM 9.97±3.38, P