INVESTIGADORES
CONTRERAS edgardo Martin
artículos
Título:
Monitoring the Enzymatic Oxidation of Xenobiotics by Hydrogen Peroxide through Oxidation–Reduction Potential Measurements
Autor/es:
MORALES URREA, D. A.; HAURE, P. M.; CONTRERAS, E. M.
Revista:
INDUSTRIAL & ENGINEERING CHEMICAL RESEARCH
Editorial:
AMER CHEMICAL SOC
Referencias:
Año: 2018 vol. 57 p. 16518 - 16525
ISSN:
0888-5885
Resumen:
In thiswork, three xenobiotics (orange II, phenol and BPA) were oxidized by hydrogenperoxide in the presence of a horseradish peroxidase (HRP) using a fed-batchsystem. During the experiments, the oxidation-reduction potential (ORP) of thereaction mixture was measured continuously. Results demonstrate that ORP valuesonly increased when both substrates of the enzyme (hydrogen peroxide and thetarget compound) were present in the reaction mixture. For all the testedpollutants, the continuous addition of hydrogen peroxide caused an increase inORP values. When the reducing substrate was depleted, the addition of an excessof hydrogen peroxide caused a decrease of ORP values. The time at which ORPreached a maximum represented the end of the oxidation process. This maximum couldbe easily detected by means of the derivative of ORP as a function of time. Toextend the application of the developed technique, the enzymatic oxidation of abinary mixture of BPA and OII was also followed using ORP measurements.  Results were similar to those observed withonly one reducing substrate. This work demonstrates that ORP measurements canbe useful to maximize hydrogen peroxide efficiency through the controlledaddition of the oxidant during the oxidation of OII, phenol, and BPA catalyzedby HRP. This approach allows minimizing time and process costs since thereaction end-point can be easily detected on a real-time basis.