HO-1 genetic variants and its effects in thyroid cancer biology

ALONSO E.G.; MASCARO M.; SCHWEITZER K.; FERNANDEZ CHAVEZ L.; COLO G.P.; ALONSO E.N.; FERRONATO M.J.; FERMENTO M.E.; ROSENBLIT C.; CURINO A. C.; FACCHINETTI M.M.

Mar del Plata

Congreso; LXVIII Reunión Anual de la Sociedad Argentina de Investigación clínica (SAIC).; 2023

Sociedad Argentina de Investigación clínica (SAIC).

In previous studies on human thyroid cancer (TC), we observed elevate levels of hemeoxygenase-1 (HO-1) protein in both cytoplasmatic and nuclear compartments. Additionally, increased HO-1 mRNA expression correlated with tumor progression. Activationof HO-1 through hemin treatment in the TPC-1 cell line promotedcell viability, proliferation, migration, and cell cycle progression,while inhibition via ZnPP had opposite effects. This study aimed toinvestigate the impact of genetically overexpressed HO-1 variants(full-length - FL, enzymatically inactive - H25A, and nuclear truncated - t-HO1) on cancer-related processes. Using stable transfections of these variants into TPC-1 cells, we observed that FL andH25A forms were predominantly overexpressed in the cytoplasm,while t-HO-1 accumulated in the nucleus. Overexpression of FL andt-HO-1 significantly enhanced cell viability (p