New Carrot Microsatellites - Linkage Mapping, Diversity Analysis And Transferability To Other Apiaceae

CAVAGNARO, P. F.; CHUNG, S.-M.; MANIN, S.; ATKINS, A. E.; SIMON, P. W.

San Diego, California

Conferencia; Plant and Animal Genomes XVIII Conference; 2010

Nearly 300 new microsatellite, or simple sequence repeat (SSR) markers were developed from genomic sequences of carrot. Efforts to map these markers and evaluate their usefulness in diversity studies are underway. In one F2 carrot population, a total of 51 polymorphic markers, including 37 codominant and 14 dominant SSRs, were placed in the reference carrot genetic map developed from a domesticated (B493) x wild (QAL) carrot cross. The mapped SSRs were distributed across all 9 linkage groups (LGs) of the carrot genome, with 3 to 9 markers/LG, recommending their usefulness for merging maps. Three SSRs in LG2 and LG5 were located in the vicinity of two highly-significant quantitative trait loci (QTL) for root carotenoid accumulation. Several microsatellites were closely linked (