Análisis de asociación en zanahorias moradas

BANNOUD, F.; ELLISON, S.; FANZONE, ML; SENALIK, D.; HOREJSI, T.; SIMON, P. W.; CAVAGNARO, P. F.

Rosario

Simposio; Simposio de Genomica Funcional de Plantas; 2017

Purple or black carrots are native to the Middle East and their purple color is due to anthocyanin accumulation. The consumption of anthocyanin-rich vegetables confers several health-related benefits, including protection against atherosclerosis and some types of cancer. Purple carrots can accumulate anthocyanins in different tissues such as roots, leaves, and flowers. A genome wide association study (GWAS) was performed to identify chromosome regions associated with total anthocyanin content in the root phloem and xylem, and leaf petioles; and with concentration of individual anthocyanin pigments in phloem and xylem. Genotyping by Sequencing (GBS) in an F2 carrot population segregating for anthocyanin pigmentation in different plant tissues generated 28,473 SNPs, which were used for association analysis using TASSEL software. Regions on chromosome 3 were significantly associated with total anthocyanin content in all three tissues. One of these regions, of ~ 1.5 Mb, was significantly associated with anthocyanins accumulation in both petioles and xylem. For phloem pigmentation, several regions on chromosome 3 were significantly associated with the trait, suggesting more than one gene controlling the expression of this trait. Ongoing studies of transcriptome analysis by RNA-Seq in purple and non-purple phloem are expected to reveal candidate genes associated with anthocyanin accumulation in this tissue.