INVESTIGADORES
CAVAGNARO pablo Federico
congresos y reuniones científicas
Título:
Genetics of tissue-specific anthocyanin pigmentation in carrot
Autor/es:
BANNOUD, F.; ELLISON, S.; FANZONE, ML; SENALIK, D.; HOREJSI, T.; SIMON P. W.; CAVAGNARO, P. F.
Lugar:
San Fernando del Valle de Catamarca
Reunión:
Congreso; XLVI Congreso Argentino de Genetica; 2017
Resumen:
Purple carrots can accumulate anthocyanins indifferent root and leaf tissues. The consumption ofthese pigments may reduce the risk of cardiovasculardisease, diabetes, arthritis and cancer, mainly due totheir anti-oxidant and anti-inflammatory activities.In this study the genetic basis of anthocyaninaccumulation in different tissues was investigated.An F2 population (N= 254) segregating foranthocyanin accumulation was visually phenotypedby scoring anthocyanin pigmentation in the rootxylem and phloem and in leaf petioles. Additionally,the content of 5 anthocyanin pigments in the rootxylem and phloem, independently, was estimatedby HPLC analysis. A linkage map was constructedusing 1014 Genotyping by Sequencing (GBS)markers, distributed across 9 linkage groups (LG),which covered 910 cM of total map length, with LGsranging from 74.2 cM (Chr 5) to 124 cM (Chr 1).Genome wide association analysis in this F2 identifiedregions associated with tissue-specific anthocyaninpigmentation in carrot root. In addition, a regionspanning ~1.5 Mbp of Chr 3 was significantlyassociated with anthocyanin accumulation in leafpetioles and the root xylem, suggesting that thesame region conditions pigmentation in both tissues.Several regions on Chr 3 were significantly associatedwith phloem pigmentation, suggesting multiple lociconditioning this trait. Ongoing QTL mappingand transcriptome analysis by RNA-Seq in purpleand non-purple phloem tissues may help to furtherdelimit the chromosome regions associated withphloem pigmentation and find candidate genes for this trait.