KH2PO4 improves cellulase production of Irpex lacteus and Pycnoporus sanguineus

RODRÍGUEZ, MARÍA DANIELA; ALONSO PAIVA, IVÁN MATHÍAS; CASTRILLO, MARÍA LORENA; ZAPATA, PEDRO DARÍO; VILLALBA, LAURA LIDIA

Journal of King Saud University - Science

Elsevir BV

Año: 2019 vol. 31 p. 434 - 444

1018-3647

The optimization of cellulase production by Irpex lacteus and Pycnoporus sanguineus was investigated.Fractional factorial design was conducted to determine significant variables and interactions. Response surface methodology was applied through Box-Behnken design to determine the optimum level of each factor on cellulase production. The optimal conditions of culture media were (g/L): for I. lacteus CaCl 2 .2 (H 2 O) 0.3, MgSO 4 .7(H 2 O) 0.3 and KH 2 PO 4 3, while for P. sanguineus was CaCl 2 .2(H 2 O) 0.1, MgSO 4 .7 (H 2 O) 0.2 and KH 2 PO 4 9. This optimized medium improved cellulase production, especially b-glucosidase activity values for I. lacteus. KH 2 PO 4 was found in this work to be a useful mineral compound for cellulolytic production.