INVESTIGADORES
CARRILLO carolina
artículos
Título:
Trypanosoma cruzi amino acid transporter TcAAAP411 mediates arginine uptake in yeasts
Autor/es:
CARRILLO C; CANEPA GE; GIACOMETTI MA; BOUVIER LA; MIRANDA MR; CAMARA MDM; PEREIRA CA
Revista:
FEMS MICROBIOLOGY LETTERS
Editorial:
WILEY-BLACKWELL PUBLISHING, INC
Referencias:
Año: 2010 vol. 306 p. 97 - 102
ISSN:
0378-1097
Resumen:
Trypanosoma cruzi, the aetiological agent of Chagas' disease, is exposed toextremely different environment conditions during its life cycle, andtransporters are key molecules for its adaptive regulation. Amino acids, andparticularly arginine, are essential components in T. cruzi metabolism. In thiswork, a novel T. cruzi arginine permease was identified by screening differentmembers of the AAAP family (amino acid/auxin permeases) in yeast complementation assays using a toxic arginine analogue. One gene candidate, TcAAAP411, wascharacterized as a very specific, high-affinity, l-arginine permease. This workis the first identification of the molecular components involved specifically in amino acid transport in T. cruzi and provides new insights for further validationof the TcAAAP family as functional permeases.