INVESTIGADORES
CARRILLO carolina
artículos
Título:
“Lack of Arginine decarboxylase in Trypanosoma cruzi”.
Autor/es:
CARRILLO C - CEJAS S; HUBER MA; GONZÁLEZ NS; ALGRANATI ID
Revista:
JOURNAL OF EUKARYOTIC MICROBIOLOGY
Referencias:
Año: 2003 vol. 50 p. 312 - 316
ISSN:
1066-5234
Resumen:
The presence of arginine decarboxylase (ADC) enzymatic activity in Trypanosoma cruzi epimastigotes is still a matter of controversy due to conflicting results published during the last few years. We have investigated whether arginine might indeed be a precursor of putrescine via agmatine in these parasites. We have shown that wild-type T. cruzi epimastigotes cultivated in a medium almost free of polyamines stopped their growth after several repeated passages of cultures in the same medium, and that neither arginine nor omithine were able to support or reinitiate parasite multiplication. In contrast, normal growth was quickly resumed after adding exogenous putrescine or spermidine. The in vivo labelling of parasites with radioactive arginine showed no conversion of this amino acid into agmatine, and attempts to detect ADC activity measured by the release of CO2 under different conditions in T. cruzi extracts gave negligible values for all strains assayed. The described data clearly indicate that wild-type T. cruzi epimastigotes lack ADC enzymatic activity.