INVESTIGADORES
ZANETTI Flavia Adriana
artículos
Título:
Vaccine strategies against Babesia bovis based on prime boost immunizations in mice with modified vaccinia Ankara vector and recombinant proteins
Autor/es:
JOSE MANUEL JARAMILLO ORTIZ; MARIA PAULA DEL MEDICO ZAJAC; FLAVIA ADRIANA ZANETTI; MARIA PAULA MOLINARI; MARIA JOSE GRAVISACO; GABRIELA CALAMANTE; SILVINA ELIZABETH WILKOWSKY
Revista:
VACCINE
Editorial:
ELSEVIER SCI LTD
Referencias:
Lugar: Amsterdam; Año: 2014 vol. 32 p. 4625 - 4632
ISSN:
0264-410X
Resumen:
In this study, a recombinant modified vaccinia virus Ankara vector expressing a chimeric multi-antigen was obtained and evaluated as a candidate vaccine in homologous and heterologous prime-boost immunizations with a recombinant protein cocktail. The chimeric multi-antigen comprises immunodominant B and T cell regions of three Babesia bovis proteins. Humoral and cellular immune responses were evaluated in mice to compare the immunogenicity induced by different immunization schemes. The best vaccination scheme was achieved with a prime of protein cocktail and a boost with the recombinant virus. This scheme induced high level of specific IgG antibodies and secreted IFN and a high degree of activation of IFNã(+) CD4(+) and CD8(+) specific T cells. This is the first report in which a novel vaccine candidate was constructed based on a rationally designed multi-antigen and evaluated in a prime-boost regime, optimizing the immune response necessary for protection against bovine babesiosis.