congresos y reuniones científicas
Humoral immune responses against Foot-and-Mouth Disease virus induced by heterologous viral vectors
ROMANUTTI CARINA; D'ANTUONO ALEJANDRA; LA TORRE JOSE; MALIRAT VIVIANA; ZAMORANO PATRICIA; QUATTROCCHI VALERIA; MATTION NORA
Puerto Madryn, Chubut.
Congreso; XLVI Reunión Anual de la SAIB; 2010
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Foot-and-mouth disease virus (FMDV) is the etiological agent of one of the most transmissible diseases of livestock, causing severe outbreaks and important economic losses worldwide. Herpes Simplex type 1 (HSV) amplicons and type 5 recombinant adenovirus (Ad) vectored vaccines were used for the induction of specific FMDV immune responses in mice. BALBc mice were vaccinated with Ad and HSV vaccines expressing FMDV structural proteins and the 3C viral protease. Three groups of mice received two sequential immunizations with HSV or Ad vectors, or 1ìg of inactivated FMDV, respectively. Homologous HSV/HSV or Ad/Ad prime/boost immunizations induced long-lasting FMDV specific antibodies. Mice immunized with HSV amplicons generated mixed IgG1/IgG2a immune responses, while immunization with Ad induced IgG2a as the predominant isotype. Challenge of mice vaccinated with HSV amplicons with a high dose of live virus, resulted in partial protection, with a significant reduction of viremia when compared to mice immunized with Ad. Heterologous vaccination regimens, priming with Ad and boosting with HSV, induced higher levels of FMDV specific antibodies than homologous prime/boost regimes. We demonstrated that Ad and HSV vectors encoding FMDV structural proteins induce potent humoral immune responses when administered either in homologous or heterologous prime/boost immunizations.