INVESTIGADORES
CLEMENTE marina
congresos y reuniones científicas
Título:
Potential functions of Arabidopsis thaliana heat shock proteins in the plant apoplast
Autor/es:
ROSSI, F.; PEREZ-ROJAS, S.; SANCHEZ LOPEZ, E.; DENG, B.; ZHANG, F.; GARRIZ, A.; CLEMENTE, M.; CORIGLIANO, M.
Lugar:
Salta
Reunión:
Congreso; LV Annual SAIB Meeting and XIV PABMB Congress; 2019
Institución organizadora:
Sociedad Argentina de Bioquimica y Biologia Molecular
Resumen:
Hsp90s are found in all plant cellular compartments examined to date, including cytosol, nucleus, endoplasmic reticulum, mitochondriaand chloroplasts. Besides, Hsp90s have also been found in the apoplast, even though their roles in this location remain elusive. We assessed theregulation of defense-associated mechanisms in Arabidopsis thaliana plants infiltrated with the recombinant Hsp90 protein rAtHsp81.2. In thistrend, we showed that cell death, ROS production and callose deposition were incremented upon rAtHsp81.2 infiltration. Besides, a 24h pretreatmentof leaves with rAtHsp81.2 resulted in a reduction in the proliferation of Pseudomonas syringae pv. tomato DC3000, whereas the invitro growth of the bacteria is not affected. Also, we observed that rAtHsp81.2 infiltration downregulated the expression of PR1 and endogenousAtHsp81.2, while Pdf1.2 expression showed no statistical differences compared to mock-inoculated controls. However, pre-treatment of leaveswith rAtHsp81.2 followed by infiltration with P. syringae or the or co-treatment (rAtHsp81.2+DC3000) provokes an opposite response.