INVESTIGADORES
CLEMENTE marina
congresos y reuniones científicas
Título:
Role of 90-kDa heat shock protein in plant immunity
Autor/es:
CORIGLIANO, M.; ROSSI, F.; CISTARI, N.; SANCHEZ LOPEZ, E.; CLEMENTE, M.
Lugar:
Cordoba
Reunión:
Congreso; LII Reunión Anual de SAIB 2016; 2016
Institución organizadora:
Sociedad Argentina de Investigación Bioquímica y Biología Molecular
Resumen:
Severalworks demonstrated that plant Hsp90s have an active role in the activation ofthe plant defense mechanisms against pathogens, since the lack or deficit ofthese proteins impairs defense against pathogenic bacteria and fungus. Hsp90sare found in all plant cellular compartments examined to date, includingcytosol, nucleus, endoplasmic reticulum, mitochondria and chloroplasts. Recently,Hsp90 have also been found in the apoplast, even though their role in thislocation has not been described. In this work, we added some light to thisissue by assessing the effects on the defense mechanisms occurring in Arabidopsis thaliana plants infiltratedwith a recombinant Hsp81.2 protein from A.thaliana (rAtHsp81.2). After 24 h, cell death, ROS and callose were accumulatedin infiltrated leaves, suggesting that the accumulation of the protein mayparticipate in the induction of defense. In this sense, a 24h pre-treatment ofleaves with rAtHsp81.2 resulted in a reduction in the proliferation of Pseudomonas syringae pv. tomato DC3000 (PstDC3000), whereas the in vitro growthof the bacteria is not affected in the presence of rAtHsp81.2. Our results suggestthat infiltration with recombinant Hsp90 is important for mounting appropriate plantimmune responses.