ISOLATION AND CHARACTERIZATION OF Lactobacillus plantarum MPL16: A WAKAME-UTILIZING IMMUNOBIOTIC STRAIN FROM SWINE FECES

Y. MASUMIZU; A. K. M. H. KOBER; B. TZU-AN CHAO; H. IIDA; W. IKEDA-OHTSUBO; Y. SUDA; L. ALBARRACIN; L. SAAVEDRA; HEBERT EM; T. NOCHI; H. ASO; T. SAITO; K. SUZUKI; J.VILLENA; H.KITAZAWA

San Miguel de Tucumán

Simposio; International Symposium on Lactic Acid Bacteria; 2016

CERELA_CONICET

Gastrointestinal infections in weaning pig are considered a major global problem with a negativeeconomic impact for livestock farmers. Several alternatives are being studied to improveresistance of pigs against gut pathogens. In this regard, a recent study has suggested that thefeeding of seaweed (wakame) is able to augment the mucosal immune functions in pigs. Onthe other hand, our group has demonstrated in vitro and in vivo that immunomodulatorylactic acid bacteria (immunobiotic LAB) are a good alternative to improve resistance againstgastrointestinal pathogens in the porcine host. Therefore, we hypothesized that a combinationof wakame and immunobiotic LAB able to metabolize and grow in this seaweed could beused as a superior functional feed to improve gut immunity in pigs. The aim of this study wasto isolate LAB from swine intestine and feces in order to evaluate: a) their capacity to utilizewakame for growing, and b) their immunomodulatory activities in porcine intestinal epithelial(PIE) cells. Isolates obtained from porcine intestine or faces belonged to the genera Lactobacillusand Enterococcus. Isolated LAB were evaluated according to their capacity to fermentwakame and positive strains were then evaluated to perform their immune-characterization.The ability of lactobacilli to beneficially modulate the inflammatory response in PIE cells afterthe challenge with enterotoxigenic E. coli (ETEC) was studied. A L. plantarum strain isolatedfrom feces, named MPL16, showed the higher capacity to modulate the production of inflammatorycytokines in ETEC-challenged PIE cells. The genome of this strain was sequencedusing a whole-genome shotgun strategy (Illumina). It consists of 3.28 Mb with mean G+C contentof 43.6%. A total of 2,880 coding sequences (CDS), 67 structural tRNAs and 13 rRNA werepredicted. Functional categories predicted by RAST showed 334 subsystems represented inthe genome. This study allowed us to identify and characterize a promising immunobioticstrain that exhibited a good potential to be used for the fermentation of wakame, and for thedevelopment of a new functional feed able to modulate gut immunity in the porcine host.