Effect of orally administered Ligilactobacillus salivarius and its CbpA mutant on TLR3-mediated intestinal immunity

SAHA, SUDEB; YAMAMURO, HIKARI; ELEAN, MARIANO; ALBARRACIN, LEONARDO; ZHOU, BINGHUI; NAMAI, FU; IKEDA-OHTSUBO, WAKAKO; NISHIYAMA, KEITA; JULIO VILLENA; KITAZAWA, HARUKI

Kyoto

Congreso; The 131st Japanese Society of Animal Science Annual Meeting; 2023

Japanese Society of Animal Science

【Objective】Ligilactobacillus salivarius modulate TLR3-mediated innate immune response in porcine intestinal epithelial cells and its surface-layer protein choline-binding protein A (CbpA) is involved in its ability to adhere and colonize the intestinal epithelium. In this study, we compared the capacity of the wild-type (WT) and a CbpA mutant (ΔCbpA) strains to modulate intestinal antiviral immunity in vivo. 【Methods】Infant BALB/c mice (3 weeks-old) were orally treated with WT or ΔCbpA strains for 5 d (108 cells/d/mouse) and then stimulated with poly(I:C) by intraperitoneal injection to induce intestinal inflammation. Non-lactobacilli treated mice were used as controls 【Results】The WT strain reduced the inflammatory-mediated intestinal damage and differentially modulated antiviral factors (IFN-β, IFN-γ, Mx1, Mx2, OAS1, OAS2, RNAsel), inflammatory cytokines (TNF-α, IL-6, IL-15) and regulatory cytokines (IL-10, IL-27) in the intestine. The ΔCbpA mutant was not efficient to modulate TLR3-mediated immunity and reduce damage. CbpA is a key factor for L. salivarius immunomodulatory properties【Acknowledgement】JSPS P22080, BRAIN 01002AB2.