The choline-binding protein A is involved in the ability of Ligilactobacillus salivarius FFIG58 to modulate TLR3-medieted intestinal antiviral immunity

YAMAMURO, HIKARI; IMAMURA, YOSHIYA; SAHA, SUDEB; JULIO VILLENA; KITAZAWA, HARUKI

Hamburg

Congreso; 10th Congress of European Microbiologists - FEMS Congress 2023; 2023

FEMS

Previous studies demonstrated that Ligilactobacillus salivarius FFIG58 adhered to porcine intestinal epithelial (PIE) cells and beneficially modulated the antiviral immune response. PIE cells treated with the FFIG58 strain had reduced expression of rotavirus proteins VP6 and NSP5 and increased expression of antiviral factors after the challenge with the pathogen. Considering that the surface-layer protein choline-binding protein A (CbpA) is involved in the ability of L. salivarius strains to adhere and colonize the intestinal tract, in this work, we advanced in the characterization of the FFIG58 strain by developing a CbpA mutant (ΔCbpA) strain and evaluating its immunomodulatory activities in vivo. Infant BALB/c mice (3 weeks-old) were orally treated with FFIG58 strain or the ΔCbpA mutant for 5 days (108 cells/day/mouse) and then received an intraperitoneal injection of poly(I:C) to induce intestinal inflammation. Non-lactobacilli treated mice were used as controls. The wild-type FFIG58 strain significantly reduced the inflammatory-mediated intestinal damage, the increase of inflammatory intraepithelial lymphocytes (CD3+CD8aa+NKG2D+ cells) and the up-regulation of pro-inflammatory mediators (TNF-α, IL-1β, IFN-γ, IL-15, RAE1, IL-8) (p