Corynebacterium pseudodiphtheriticum modulates TLR3-triggered innate immune response in bronchial epithelial cells

RAYA TONETTI, FERNANDA; SACUR, JACINTO; FUKUYAMA, KOHTARO; ELEAN, MARIANO; KITAZAWA, HARUKI; JULIO VILLENA

San Luis

Congreso; LXXI Reunión Anual de la Sociedad Argentina de Inmunología (SAI); 2023

Sociedad Argentina de Inmunología

Nasally administered Corynebacterium pseudodiphteriticum 090104 enhances the resistance of infant and adult mice to the challenge of the respiratory syncytial virus. In order to advance in the knowledge of the mechanisms involved in the beneficial effects of the 090104 strain, this work aimed to evaluate the effect of C. pseudodiphteriticum on the immune response of bronchial epithelial cells (BECs) triggered by the activation of TLR3. For this purpose, porcine BECs (1.8 104 cells) were stimulated with C. pseudodiphteriticum 090104 (Cp) (107 cells) for 48 h, and then challenged with poly(I:C) for 24h. Samples of mRNA were taken before (0h) and 3, 6, 12 and 24 h after TLR3 activation, for the evaluation of type I and III interferons (IFNs), antiviral factors, inflammatory and regulatory cytokines as well as pattern recognition receptors and negative regulators of the TLR signaling. BECs non-stimulated with Cp and challenged with poly(I:C) were used as controls. The stimulation of BECs with Cp significantly increased the expression of IFN-β, α, λ1, and λ3, along with Mx1, Mx2, OAS1, OAS2, and OASL (p