PROBIOTIC LACTOBACILLUS: PROMISING WEAPON TO AMELIORATE DISORDERS ASSOCIATED TO INTESTINAL INFLAMATION

MARTINEZ MONTEROS, MARIA JOSÉ; YAHFOUFI N; BALCELLS, FLORENCIA; PERDIGÓN, GABRIELA; MALDONADO GALDEANO, CAROLINA; CAZORLA, SILVIA INES

San Miguel de Tucumán

Congreso; LXVII Reunión Anual de la Sociedad Argentina de Inmunología; 2019

We have demonstrated that lactobacillus administration increases Paneth cells and intestinal antimicrobial peptides (AMPs) in healthy mice.Our aim was to explore whether probiotics can restore the AMPs production in intestinal inflammation. Mice were fed with: Lactobacillus casei CRL 431 (Group 1); Lactobacillus paracasei CNCM I- 1518 (Group 2) for 7 and 5 days, respectively, or water (Group 3 and 4). Intestinal inflammation was induced in G1, 2, and 3 by 7,5 mg/kg/day of indomethacin (I) on the last 2 days. G4 received injections of PBS. On day 8 mice were killed and samples were removed.A significant body weight loss, anemia, shortening in the length of the small intestine and intestinal villi, mononuclear cells infiltration of the lamina propria and decrease in Paneth and Goblet cells were observed in G3 concerning control. Probiotics supplementation in intestinal inflammation induce only a slightly modification of these parameters. Interestingly, the antimicrobial activity of the intestinal fluids of G2 and G3 against Salmonella Typhimurium,Staphylococcus aureus, and the multi-resistant drugs?: Klebsiella pneumoniae and Enterobacter aerogenes were higher regarding G3 and G4 (p