INVESTIGADORES
MALDONADO GALDEANO Maria Carolina
congresos y reuniones científicas
Título:
Probiotic lactobacillus increase the microbicidal activity of the intestinal fluids in Inflammatory Bowel Disease.
Autor/es:
MARTINEZ MONTEROS, MARIA JOSÉ; PERDIGÓN, GABRIELA; MALDONADO GALDEANO, CAROLINA; CAZORLA, SILVIA INES
Lugar:
Mar del Plata
Reunión:
Congreso; Reunión Conjunta de la Sociedad Argentina de Investigación Clínica (SAIC), la Sociedad Argentina de Inmunología (SAI) y la Sociedad Argentina de Fisiología (SAFIS); 2018
Resumen:
We have recently demonstrated that probiotics oral administration increase the number of Paneth cells, the main intestinal cell responsible of antimicrobial peptides (AMPs) production. The aim of this work was to explore, in a mouse model of inflammatory bowel disease (IBD), whether lactic acid bacteria can modify the altered production of AMP reported in the disease, in order to preserve intestinal barrier. IBD were induced in Balb/c mice by two subcutaneous doses of 7.5mg/kg/day Indomethacin, on days 5 and 6. Animals were supplemented with: Group 1: Lactobacillus casei CRL 431 (LC 431), G2: Lactobacillus paracasei- CNCM I-1518 (LC 1518) or G3: water, upon 7 and 5 days, respectively. Additional animals fed with LC 431, LP 1518 or water, respectively, received 2 injection of PBS. On day 8, mice were sacrificed. A 2.27± 1.32% body weight loss was observed in G3 after indomethacin injection, while a gain of 1.30±0.98, and 2.14±0.80 was observed for G1 and G2, respectively, and 3.79 ±1.84 for control animals. Additionally, the appearance of each mouse were analysed, registering a Score: 3 (smooth Coat) for G1 and G2, and Score: 1 (majority of back is piloerected) for G3. In vitro, an increase in the microbicidal activity against S. aureus and S. thyphimurium were observed in the intestinal fluids of G1 and G2 comparing those animals treated with indomethacin that received water in their beverage (G3). Hematoxylin-eosin studies showed severe mononuclear cell infiltrates in the lamina propria inflammatory focis and thickening of small intestinal on G3. Besides, serum markers of inflammatory process (LDH and PCR) were increase in G3 comparing with G1 and G2 (p˂0.05). Finally, a slightly increase of total anaerobes and lactobacillus bacteria and decrease of total enterobacteria were observed as part of the colon microbiota in G1 and G2 animals compared to G3.Probiotics can be promising microorganisms in the stimulation of intestinal antimicrobial activity in pathologies such as inflammatory bowel disease, contributing to preserved intestinal barrier, combat microbial pathogens and regulate the intestinal immune system.