INVESTIGADORES
MALDONADO GALDEANO Maria Carolina
congresos y reuniones científicas
Título:
MECHANISMS BY WHICH LACTOBACILLUS CASEI CRL 431, A PROBIOTIC MICROORGANISMS, INTERACTS WITH THE GUT IMMUNE CELLS TO INDUCE IMMUNE RESPONSE
Autor/es:
MARIA CAROLINA MALDONADO GALDEANO; PERDIGÓN, G.
Lugar:
Boston, Massachusetts
Reunión:
Congreso; 12th Internacional Congress of Mucosal Immunology; 2005
Resumen:
Background: We demonstrated that small fragments of probiotic bacteria make contact with the immune cells present in the intestinal mucosa. This interaction produces immune stimulation with increase in the number of cytokines and IgA producing cells. Methods: We investigate how these antigenic particles interact with the cells of the innate immune response of the gut, to initiate the cell activation and whether or not the adaptative response is influenced. We determined the macrophages and DC receptors (CD206 and TLR2) and T lymphocytes markers (CD4, CD8 and CD3). BALB/c adult mice were fed in the drinking water with Lactobacillus casei (Lc) CRL 431 (108 CFU/day) during 2, 5 or 7 days. At the end of each feeding period the animals were sacrificed. The small intestine was removed for histological slices preparation. We measured the expression of the receptors: CD206, TLR2, CD4, CD8 and CD3 by immunofluorescence. CD206 and TLR2 receptors were also determined by immunoperoxidase assay, in immune cells isolated from Peyer’s patches. Results:  The study of the different receptors in the lamina propria from small intestine, showed an important increase for all days assay in the number of mannose receptor CD206 present in macrophages or DC surface as well as in the number of TLR2 in comparison with the unfed control.  Similar results were observed in the immune cell isolated from Peyer’s patches. T lymphocytes population was not increased. Conclusions: The results demonstrated that the interaction of Lc fragment with the immune cells from lamina propria and Peyer’s patches produced an important activation of the innate immune response evidenced for the increase of the receptors present in the macrophages or DC surfaces .While there was not proliferative increase of T lymphocytes which are linked with the adaptative immune response. The innate immunity would be more involved in the immune stimulation induced by this probiotic microorganism.