PROBIOTICS INCREASE ANTIMICROBIAL ACTIVITY OF PANETH CELLS IN ELDER MICE

CAZORLA, SILVIA INES; MARTINEZ MONTEROS, MARIA JOSÉ; MALDONADO GALDEANO, CAROLINA; PERDIGÓN, GABRIELA

Congreso; Reunión Conjunta de Sociedades de BioCiencias; 2017

The number of bacteria is very low in the small intestinal tract. This seems to be, at least in part, to the abundant constitutive antimicrobial peptides (AMPs) which are expressed by crypt Paneth cells. Our aim, using mice as experimental model, was to explore whether probiotics can strengthen intestinal barrier through the life of the animals, by increasing the microbicidal activity.BALB/c mice from 21, 28, 35, 42, 54, 61 and 180 days old, received L. casei CRL 431 (Lc 431), L. paracasei CNCM I-1518 (Lp 1518) or water (control), upon 7 and 5 days, respectively. After, mice were sacrificed and intestinal fluids, small and large intestine sections were taken. Forty two days old mice, fed with Lc 431 or Lp 1518, increased the number of positive Paneth cells (64.29 ± 3.58 and 61.80± 5.31%, respectively), respect to control (40.10 ± 3.9%) (Mean ±SEM). Weaning mice (21 days old) that received Lc 431 or Lp 1518 did not show a significant microbicidal activity in the intestinal fluids. In contrast, an important reduction in the CFU of S. aureus and S.Typhimurium were observed at 35, 42, 54 and 61 days old (p