Cell Wall from Lactobacillus casei CRL431 Induce both Intestinal Epithelial and Macrophage Cells Activation

LEMME DUMIT, JOSÉ MARÍA; PERDIGÓN GABRIELA; MALDONADO GALDEANO, CAROLINA

Berlin

Congreso; ICMI 2015 17th International Congress of Mucosal Immunology; 2015

Society for mucosal Immunology

Cell wall (CW) molecules from probiotic are crucial to interact through host-receptors and induce signaling that result in theimmune system modulation. The knowledge of bacterial components as immunomodulators offers interesting opportunities toelucidate a novel structure as oral adjuvant. In the current study we aimed to study the impact of CW from Lactobacillus caseiCRL431 (Lc431) in the activation of intestinal epithelial cell (IEC) and peritoneal macrophages (MQP) to be the sentinels of innateimmune cells. CW fraction was obtained from Lc431 (CW431). Ex vivo, phagocytic and antimicrobial activities of MQP weredetermined. In vitro, IL-6 levels of supernatant of MQP were determined. IEC activation was observed trough transmissionelectron-microscopy (TEM). Lc431 and CW431 groups showed phagocytic and microbicidal activity significantly higher in thosegroups when compared with the control mice, while values for IL-6 do not improved respect to control group. However, Lc431 andCW431 showed for TEM a higher activation of IEC after 30 min of stimulation when compared with IEC control. Probiotic are able toactivate and improve the activity of IEC that is a key cell in mucosal immune system activation. This work demonstrated that cellwall fraction, could be interesting alternative as oral mucosal immunomodulators.